事实
与Levitronix® 泵相比,4 活塞隔膜泵由多个阀门组成,可将流体运动限制在所需方向。 阀门相互作用会导致剪切应力,从而导致细胞存活率降低。
测试条件
通过观察它们对各自细胞活力的影响,比较了两个 Levitronix® 和一个 4 活塞隔膜泵的剪切应力产生。 在三个相同的闭环配置中以泵輸入固定体积的 CHO 细胞。 每个泵的流速分别设置为 3.4 lpm 和 0.03、0.3 和 0.6 bar 的背压。 在选定的时间测量细胞存活率并进行比较。
结果
与 4 活塞隔膜泵相比,两种Levitronix® 泵系统都显示出对 CHO 细胞的细胞应激效应显着降低。 即使在高速下,Levitronix® 泵的细胞死亡率也比 4 活塞隔膜泵低约 30%。
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